62°C vs 64°C annealing in primertests

1 Define Criteria for Primer Acceptance based on the data from the primertests

1.1 ΔCT

   

   

1.2 Sample CT

   

   

1.3 BC CT

   

   

1.4 Δ RN

   

Assays that fulfilled the above criteria (SignalCT < 31.72 & BC_CT > 37.03 & deltaRN > 7.1 & dCT > 8.94 ) predicted as accepted, otherwise as rejected.

   

2 Sankey plots for annealing 62°C vs 64°C and primer acceptance based on the above criteria

2.1 All rearrangement classes

 

2.2 Sankey plots by Rclass

2.2.1 VhJh

 

2.2.2 DhJh

 

2.2.3 VkJk

 

2.2.4 VkKDE

 

2.2.5 intronKDE

 

2.2.6 VbJb

 

2.2.7 DbJb

 

2.2.8 VgJg

 

2.2.9 VdJd

 

2.2.10 VdDd

 

2.2.11 DdDd

 

2.2.12 DdJd

 

2.2.13 VdJa

 

2.2.14 DdJa

3 Real acceptance 62 vs 64

Based on the akzeptiert value from LIMS

3.1 All rearrangements classes

3.2 By rearrangement class